Ramification of hcg trigger

So my second line is gone.  The ramifications of hcg trigger.  I've been secretly peeing on stick since the iui, so I could watch it fade and know exactly when a positive would be a positive (shhhhh- don't tell my mom; I told her I wasn't obsessing).  Haha look who I have fooled.  Seriously, hoping this second line comes back.